The chamber. Access this by going to Help >. A wireless light sensor (701-999, PS-3212) was purchased from PASCO. . Monday to Friday 8am - 5pm GMT. EUROPE. 303 unable to cal dark current. Cytokines in medium released. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Molecular Devices LMax II Luminometer Luminescence Microplate Reader w WARRANTY $2,450. Neutrophils from six HC patients were stimulated by PMA with or without pretreatment of SIRL-1 mAb, and then co-cultured with autologous monocytes for 12 h. Devices SpectraMax i3, SpectraMax Paradigm, or SpectraMax M5 Multi-Mode Microplate Readers. The drawing shows a standard microplate of 15 mm height covered with a lid. In other words, I carry out equilibrium folding/unfolding titrations of proteins and monitor the process using. 0 mL). The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial numbers. spr * SpectraMax i3(x) SpectraTest ABS2 v1. Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. Optical absorbance was measured at 570 nm on a SpectraMax M5 microplate reader. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. . Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Offer Ends In: 18 Days 19: 38: 21. . It does not work as expected. Spectral Range. The BenchCel Microplate Handling Workstation provides microplate-orientation sensing so all microplates enter the reader in the same orientation, ensuring that the data output is consistent from the first microplate to the last. 306 plate air cal. The PathCheck sensor can extend the instrument linearity by a factor of three or more. SpectraMax® M5 Multi-Mode Microplate Reader (Molecular Devices P/N #M5) SpectraMax® Gemini™ EM Microplate Reader (Molecular Devices P/N #EM) Methods Instrument and protocol setup. HTRF-compatible SpectraMax Multi-Mode Readers. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuidePlace the plate into a SpectraMax M5 e plate reader (Molecular Devices). Features. Streamline BCA-based protein quantitation on the SpectraMax iD5 reader. . the IMAP Platform and smoothly transition into high-throughput screening. S. Molecular Devices Spectramax M5 luminometer: Krishnamurthy lab-Praveen (lab WO3213, x4605) Spectramax plate reader: yes Beckman LS6000IC Scintillation counter: Bill Taylor (office WO4262B, x1966) WO3242 liquid scintillation counter Department backup -80oC freezer: Song-Tao Liu (WO4254, x7857) Facilities Facility: Department. 1) Dual Luciferase Reporter Assay (FlexStation) SpectraMax Glo Steady-Luc Reporter Assay; EarlyTox. 3: The cuvette chamber. 5. Add to Cart. We would like to show you a description here but the site won’t allow us. HTRF is a proprietary time-resolved fluoresce nce technology that overcomesand emission wavelengths for SpectraMax M5 fluorescence microplate readers are as follows: excitation at 490 nm and emission at 525 nm, with 515-nm emission cutoff filter (note that these are different from the wavelengths recommended for filter-based readers in the Quant-iT PicoGreen product insert). Quantitate proteins rapidly with a 5-minute, room-temperature incubation. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. $1,250. Lower limits of detection for ATP determined using the ATPlite . The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. These robust readers have been placed in labs from Antarctica to the. The. The following document is intended to demonstrate setup of this instrument and provide representativeThe SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro Data Acquisition and Analysis Software. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. The SoftMax® Touch Software is installed in. 3. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. The microplates were read the next day from the bottom and the top in SpectraMax M5 and Gemini EM readers. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm pathlength cuvette. D 1 1. Both full length human PI4KIIIB and the truncated PI4KIIIB crystal construct with the c-term were run at 200 nM. . SpectraMax®L Luminometer User Guide 0112-0174 B 11 The SpectraMax L Luminescence Microplate Reader can accommodate SBS-standard 96 and 384-well microplates with dimensions of 128. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. Step 2. M5. . Cet appareil ISM est confomre à la norme NMB-001 du Canada. together with SpectraMax, VersaMax, VMax, and EMax microplate readers from Molecular Devices, offer a quality assurance screening solution for the detection of melamine contamination in such food products as milk, infant formula, pet food, and confectionery. Pick the best speed, sensitivity, and price for your needs. Note: In this user guide, SpectraMax ® refers to several SpectraMax . Open the Softmax Pro software and select the FP option. free tracer decreases, resulting in an. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. Wypalls Paper Towel (Warehouse, Cat # 79300335 or equivalent) 7. 4. The following document is. Wavelength Range. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-Mode We would like to show you a description here but the site won’t allow us. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. . The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. 3. ORDER. Figure 2. The excitation and the emission wavelengths on. In either case. . When used with SoftMax® Pro Data Acquisition and Analysis Software, it can automatically normalize the well absorbance to a cuvette equivalentAnalytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). SpectraMax ABS Plus SpectraTest ABS2 v1. Recertification is recommended every two years. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. clicked with the SpectraMax L Microplate Reader. 1. Two sequential measurements should be carried out: at 620 nm for the cryptate emission, and at 665 nm for the specific signal emitted by the acceptor. 2. View the Molecular Devices websiteResults by SpectraMax M5 from multiple independent donors are given. SOFTmax PRO UserÕs Manual iii Contents Figures . The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. . Refer to “Installing Detection Cartridges” in. ISM Equipment Classification (Group 1, Class A) This equipment is. For more information, please visit us at Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Description 1. you can use a resource. Softmax Pro GxP Software 6. Multimode Readers Comparison Chart. Table 1. In this. Formula accessors for reporting o The accessor!sectionname will return the name of the SoftMax Pro section containing the. High tolerance for contaminants. • Tel. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. Description 1. The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. Molecular Devices ThermoMax Microplate Reader COMPLETE w WARRANTY $1,750. Molecular Devices Spectramax M5 Plate Reader is available from The Lab World Group. SpectraMax® M5/M5e Multimode Plate Reader, Molecular Devices Supplier: Molecular Devices Description: The SpectraMax® M5 Multi-Mode Microplate Reader delivers single. The drawing shows a standard microplate of 15 mm height covered with a lid. (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. Softmax Pro GxP Software 6. Description. 4. 1. 72 MB. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. Molecular Devices ®(MD) SpectraMax 340PC reader MD VersaMax™ Tunable Microplate readers MD SpectraMax® L Microplate Luminometers MD SpectraMax® M5 systems MD ®SpectraMax 96/384-well plate reader Gemini fluorescence plate reader Automation Hamilton Microlab® STAR™ liquid handling systemThe SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy • Emission wavelength accuracy • Excitation wavelength precisionHere, we present FluoroBrite™ DMEM, a cell culture media designed specifically for live-cell imaging that, when excited at wavelengths commonly used for FITC/GFP (i. . 0 mm wide, and heights from 14 mm to 15 mm. We would like to show you a description here but the site won’t allow us. Choose from a three- (M3), four- (M4), or Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. 6: bioNova científica, s. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. . These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. This version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. 005) Photometric PrecisionThe Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well. The large analog PMT works with monochromators and is used during fluorescence measurements or when a specific luminescence wavelength is selected as in the BRET. Fluorescence intensity was measured using a. G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax i3x, SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 Linearity G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax. Microplate Readers Biotek Synergy H1 Hybrid Microplate Reader. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Proceed with the following steps to optimize the instrument: 1. the IMAP Platform and smoothly transition into high-throughput screening. Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. 检测模式包括： • 紫外-可见光 光吸收 (Abs) • 荧光强度 (FI) • 化学发光 (Lum) • 时间分辨荧光 (TRF) • 荧光偏振 (FP) SpectraMax M5e 在 M5 基础上通过了 Cisbio HTRF 检测认证而增加了应用范围。. 314 low lamp level warning. Call 516-939-0888 to check current. 0 out of 5 $ 15,999. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. D 1. iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology:. 72 MB. There were 12 replicate wells per dilution in both 96-well and 384- well plates. 2. Five preferred modes for diverse bioresearch applications. . Choose cables that meet the requirement described in this appendix. SIRL-1 ligation reduced cytokines secretion from monocytes stimulated by NET. 1. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The following document is intended to demonstrate setup of this instrument. Setup for Z’-LYTE® Assay on SpectraMax® M5/M5e Microplate Reader with SoftMax® Pro 6 Software. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. D 1. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. The dual monochromator optics allow the widest range of applications to be utilized for bior. . . TR-FRET assay. Figure 4: Transcreener ADP. Bookings are free, but you will need to use iLab to book. The KeySpan USA-19HS is often required with new Windows 7. Step 4: Incubate at room temperature for 1 hour, protected from light. General procedure forpreparedviscosity analysis The parent stock solution was prepared by dissolving probe FCV (5 mM) in DMSO. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. The hydrolysis was monitored continuously by top-read fluorescence using a Spectramax M5 multimode plate reader (Molecular Devices, USA) from the release of 7-amino-4-methylcoumarin (AMC) using an excitation wavelength of 380 nm and monitoring the emission at 460 nm over 1 h. SpectraMax Spectrofluorometers. Spectranet customer portal. HEALTH AND SAFETY CONSIDERAIONS The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. The REF button has no valid meaning for. Description 1. Microplate Readers Molecular Devices Spectramax M5 Microplate Reader. Description 1. +44-118-944-8000. . edu) to be added as a trained user in iLab. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. The absorbance of MPO was calculated using the SpectraMax M5 (Molecular Devices Corporation, San Jose, CA, USA) at 460 nm. When the user applies a set temperature of 37°C, the top is heated to 37. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. . 7°C (gradient heating design). Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. Features. Molecular Devices SpectraMax M5e User Manual (86 pages) Multi-Mode Microplate Readers. The SpectraMax M5 Multi-Mode. HEALTH AND SAFETY CONSIDERAIONSThe Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. Table of Contents. The Spectramax performs spectral scanning in 1 nm increments, and up to six wavelengths. 5. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideIn this application note, we demonstrate the measurement of luciferase expression in CHO-K1 cells using the SpectraMax Glo Steady-Luc Reporter Assay Kit, which affords long-lasting luminescence signals. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. Table of Contents. $4,499. Select the fluorescence option and read the total fluorescence. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. The SpectraMax M5e Microplate Reader enables five modes of detection with the additional benefit of being certified for Cisbio Bioassays’ HTRF Assays. e. . Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15 Monochromator Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. The time course of the cleavage reaction was monitored by loss of FRET (excitation, 450 nm; emission, 655 nm) with 15 reads/well and 4–5-s read intervals on a SpectraMax M5 plate reader (Molecular Devices). The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested. 9000-0756. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. How do I set up Hellma Cuvette validation set recertification? Send Hellma cuvette standards to Hellma. New drivers take 6 days to write, existing drivers take 2 days to convert to Windows 7/10 compatibility. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well SpectraMax M5 Multi-Mode Microplate Reader. Multimode Reader Comparison Chart. 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax Microplate Stacker for walk-away processing. The plastic from a microplate will affect the light polarization, precluding bottom reads and reading a covered plate. 7°C and the bottom to 36. 95. individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). Dual monochromators provide the ability for. If you do not have an iLab account you must first make one: SpectraMax Paradigm Fluo rescence Polarization (FP) Detection Cartridge is a single slot detection cartridge and occupies one (1) slot. 4. MDS Analytical Technologies announced the launch of the Molecular Devices® SpectraMax® M3 and M4 Multi-Mode Microplate Readers. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Reduces well-to-well variability, improving assay quality (Z' factor) and reliability (CV %) of high-throughput. Setup recommendations for SpectraMax® M5e. Example: [Drawer]. Flexible for 6-well to 384-well microplates. Products : SoftMax Pro GxP Software, SoftMax Pro Software, SpectraMax M5/M5e reader. Third this recommendation. Molecular Devices Spectramax M5 luminometer: Krishnamurthy lab-Praveen (lab WO3213, x4605) Spectramax plate reader: yes Beckman LS6000IC Scintillation counter: Bill Taylor (office WO4262B, x1966) WO3242 liquid scintillation counter Department backup -80oC freezer: Song-Tao Liu (WO4254, x7857) Facilities Facility: Department. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. 3: The cuvette chamber. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Microplate Reader Shelf. The SpectraMax ABS Plus reader utilizes Molecular . edu) to be added as a trained user in iLab. This application note describes how to. Absorbance (UV – VIS) Fluorescence; Glow luminescence; Time-resolved fluorescence; Fluorescent polarization; ABI Viia7 qPCR instrument (96 format) – SYBR/FAM/VIC/ROX calibrated. Figure 1. e. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. . The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. The hydrolysis was monitored continuously by top-read fluorescence using a Spectramax M5 multimode plate reader (Molecular Devices, USA) from the release of 7-amino-4-methylcoumarin (AMC) using an excitation wavelength of 380 nm and monitoring the. 313 reference gain check fail. 305 reference level saturation. Italic and boldface type are used for emphasis. TNF-alpha quantification down to low picomolar levels on the SpectraMax Paradigm Platform. If you do not have an iLab account you must first make one: SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability and also uses patented PathCheck® Pathlength Measurement Technology – the only temperature independent pathlength correction for microplates. Molecular Devices Corporation have recently introduced the SpectraMax® M5e multi-detection microplate reader, an extension of the five-mode SpectraMax M5 system. D 1. Choose from a three- (M3), four- (M4), or SpectraMaxM3,M4,M5,andM5eMicroplateReaderUserGuide 26 0112-0115J ApplicationsofAbsorbance Absorbance-baseddetectioniscommonlyusedtoevaluatechangesincolororturbidity,. View the Molecular Devices websiteSpectraMax® M5 multimode microplate reader from Molecular Devices to measure both reporters in a completely homogeneous manner, in the same well of a 96-well plate, without cell lysis. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Next, an AlphaLISA assay was developed to detect levels of pro-inflammatory factors produced by endothelial cells in response to TNF-alpha stimulation. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. ItSpectraMax® M5多功能读板机为使用者提供了除化学发光以外的其他检测方法，例如：光吸收，荧光强度，荧光偏振和时间分辨荧光。 SpectraMax® L和SpectraMax® M5酶标仪均用SoftMax® Pro软件进行操控和设置，该软件含有CellTiter-Glo检测模板和其他120多个分析模板，这些. Data Acquisition & Analysis Software. Highly selective for dsDNA over ssDNA or RNA. Molecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Terbium-based TR-FRET assays. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode…. 5. Methods Preparation of reagents and standards. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. Choose from a three- (M3), four- (M4), orThe SpectraMax M5, M5e, and FlexStation 3 Multi-Mode Microplate Readers have been validated for use with the ValitaTiter and ValitaTiter Plus assays. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. The following document is intended to demonstrate setup of this instrument. Wavelength optimization. SpectraMax® Microplate Readers and SoftMax® Pro Software. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. LMax, SpectraMax ®, VersaMax™, VMax , EMax , UVMax, FlexStation®, and ThermoMax benchtop Microplate Readers. Fluorescence Microplate Reader Comparison Chart. The plate reader. Add to Cart. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). Efects of luciferase concentration, luciferin concentration, ATP concentration, pH, temperature, and reaction time on the. Detection of pro-inflammatory factors. Control Panel The control panel houses the instrument status LEDs and the microplate. . 2. 312 gain calibration failed. it is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax® Microplate Stacker forF1 SpectraMax Plus384andABSPlus: Certificate Value – 2≤ Average Peak Value≤ Certificate Value + 2 SpectraMax M2,M2e,M3,M4,M5,andM5e: Certificate Value – 3≤ Average Peak Value≤ Certificate Value + 3 Photometric Accuracy F2, F3, F4 AverageOD=Certificate Value± (Certificate Value(0. 3: The cuvette chamber. Step 4. OptimizingAbsorbanceReadMode The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. Offer Ends In: 18 Days 19: 38: 21. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. 00 USD. 0 out of 5 $ 17,999 $ 16,995. 7. . SpectraMax Microplate Readers. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. Microplate Reader Shelf. Wypalls Paper Towel (Warehouse, Cat # 79300335 or equivalent) 7. ability to detect 16 ng/well of DNA and to quantitate 50 ng of . Consult Molecular Devices's entire SpectraMax M series catalogue on MedicalExpo. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. 2. 190-1000 nm, tunable in 1 nm increments. . The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Data collected by Analyst® and FLIPR® Systems can also be imported and analyzed in the software. Fluorescence spectra of CDs were measured using a microplate reader (SpectraMax M5, Molecular Devices). Bio-Tek BioTek uQuant Microplate Reader Complete w WARRANTY $3,450. 190 - 1100 nm. Open the SoftwarePro® Data Acquisition & Analysis software,. SpectraMax Injector Cartridge with SmartInject™ Technology. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. . ; India imports most of its Spectramax from United States, China and Austria and is the largest importer of Spectramax in the World. Sensitive fluorescent quantitation of DNA with the Quant-iT. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini EM reader, SpectraMax M5/M5e reader, FlexStation 3 reader, SpectraMax M4 reader Which is better: top-read or bottom-read? You can use either. Or, with the reader specifications noted from the Instrument Description section of your reader's manual, as well as those for your PC, you can calculate needs more closely. The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability and also uses patented PathCheck® Pathlength Measurement Technology – the only temperature independent pathlength correction for microplates. 0 (IBM Corporation, Armonk, NY, USA). The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Bookings are free, but you will need to use iLab to book. Small, unbound molecules rotate rapidly in solution (top), while large,. . For example, microplates containing different samp les can be read using the same orPlates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. Lift up the lid over the chamber to insert or remove a cuvette. . The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplate. Issue: The audit trail in a SoftMax Pro GxP Software data file records a user as logging out of the system when the Auto Log Off prompt. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. View regional contact information and Technical Support hours: Contact Us Hi Guest, welcome to. It should work on the Spectramax L reader and also on the Spectramax M3, M4, and M5 plate readers (new firmware only) with Softmax Pro ® software version 5. Molecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Terbium-based TR-FRET assays. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. SpectraMax M5 Multi-Mode Microplate Reader. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a microplate. SPECTRAmax PLUS Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax™ PLUS microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific pro-teins, nucleic acids, and other molecules across a wide range of concentrations. Spectral Range. 多功能酶标仪 FlexStation3 快速动力学操. 0 out of 5 $ 5,999. Overall, SpectraMax M5 is a powerful lab tool that combines advanced detection capabilities, user-friendly software, and comprehensive accessory options. My experiments primarily involve monitoring the change in tryptophan fluorescence with time, using 96-well black plates. Consistent results are obtained over time offering workflow flexibility. I have been using the SpectraMax M5 since 2011. Select the fluorescence option and read the total fluorescence. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). . Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15 Monochromator Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. used. The Spectramax can accommodate cuvettes or a variety of 6 to 96-well plate formats. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. 4. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. Table of Contents. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. Examples: “Press carefully to engage,” “ Do not press down. UV/Vis absorbance and fluorescence spectra were measured on SpectraMax® M5 Multi- Mode Microplate Reader. Category 2 and 3 microplate readers use gradient heating as well. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. 3. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. ” SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a microplate. The Spectr. The SpectraMax ® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. BRET does not work on the Spectramax M5 due to a sensitivity issue and to the fact that the BRET assay has very weak signal in general. HEALTH AND SAFETY CONSIDERAIONS StakMax Handling Stacker Base Unit. . Cavicide (Warehouse, Cat # 79300360) 6. The standard curve was plotted using a log-log curve fit in the SoftMax® Pro Software (r2 = 1. Operated fromFluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay.